Glycyl-L-glutamic acid is a dipeptide composed of glycine and L-glutamic acid linked via a peptide bond. It is a white to off-white crystalline powder with the molecular formula C7H12N2O5. This compound is a specific substrate for the enzyme dipeptidyl peptidase IV (DPP-IV), which plays a crucial role in glucose metabolism by inactivating incretin hormones. Its primary application is in biochemical and pharmaceutical research as a standard or substrate for studying DPP-IV activity. Inhibition of DPP-IV is a validated therapeutic strategy for type 2 diabetes, making this dipeptide a valuable tool in drug discovery and enzymatic assay development. It is also used in peptide synthesis as a building block. As a protected form of glutamic acid with a free N-terminus, it serves as a versatile intermediate in solid-phase peptide synthesis (SPPS) for constructing longer peptide sequences. It should be stored in a cool, dry place and handled with standard laboratory precautions for fine chemicals.