HEPES, CAS No. 7365-45-9

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7365-45-9

Catalog No. EBD36748

CAS 7365-45-9

Name HEPES

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Basic Information

Synonyms: 4-(2-hydroxyerhyl)piperazine-1-erhanesulfonicacid 4-(2-Hydroxyethyl)-1-piperazineethanesulphonicacid N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid) HEPES N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid),TissueCultureTested N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid),MolecularBiologyGrade 4-(2-hydroxyethyl)-1-piperazineethanesulfonicacid 4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONICACID 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONICACID LABOTEST-BBLT00138109 LABOTEST-BBLT00233156 2-[4-(Hydroxyethyl)-1-piperazinyl]ethanesulfonicacid 2-[4-(2-hydroxyethyl)piperazin-4-ium-1-yl]ethanesulfonate 2-[4-(2-hydroxyethyl)piperazinediium-1-yl]ethanesulfonate N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonicacid) 4-(2-Hydroxyethy1)-1-piperazineethanesulfonicacid N-(2-Hydroxyethyl)-piperazine-N'-2-ethanesulfonicacid N-2-Hydroxyethylpiperazine-N-2-ethanesulfonicacid 4-(2-Hydroxyethyl)-1-piperaz

Molecular Formula: C8H18N2O4S

Molecular Weight: 238.3

MDL Number: MFCD00006158

Categories: Medicinal Chemistry > Pharmaceutical Excipients > Isotonic Agents, pH Adjusters, and Buffers

Product Description:

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent. Its structure features a piperazine ring substituted with a hydroxyethyl group and an ethanesulfonic acid group, giving it a pKa of approximately 7.5 at 25буC. This makes it an excellent buffer in the physiological pH range (6.8-8.2), with minimal metal ion binding and negligible interference with biological reactions. Its primary application is as a non-toxic, non-reactive buffer in cell culture media, particularly for maintaining pH stability in CO_-independent systems. It is also widely used in biochemical and molecular biology assays, including enzyme kinetics, protein purification, and electrophoresis, where consistent pH is critical. HEPES is preferred over phosphate buffers in many applications due to its low chelating ability and stability. As a general-purpose biological buffer, HEPES is a standard reagent in life science research and diagnostic kits. It is also utilized in some industrial formulations, such as in vitro diagnostic products and cosmetic preparations, where pH control is required. Its high purity and consistent performance make it a staple in laboratories worldwide.

Physical Properties

Melting Point: 234-238 °C

Density: 1.07 g/mL at 20 °C

Solubility: H2O: 1 M at 20 °C, clear, colorless

Refractivity: n20/D 1.339

Storage: 2-8°C

Analytical Data

Appearance: White crystalline powder