Succinimidyl 6-(biotinamido)hexanoate, CAS No. 72040-63-2

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72040-63-2

Catalog No. EBD13551

CAS 72040-63-2

Name Succinimidyl 6-(biotinamido)hexanoate

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Basic Information

Synonyms: Biotin-C5-NHS Ester BiotinamidohexanoicacidN-hydroxysuccinimideester N-SuccinimidylN-biotinyl-6-aminocaproate N-(+)-Biotinyl-6-aminocaproicacidN-succinimidylester (+)-BiotinamidocaproateN-hydroxysuccinimidylester Succinimidyl6-(biotinamido)hexanoate N-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide N-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide N-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanami

Molecular Formula: C20H30N4O6S

Molecular Weight: 454.54

Categories: Medicinal Chemistry > Biochemicals and Life Science Reagents > Fluorescent Probes and Labeling Reagents Medicinal Chemistry > Biochemicals and Life Science Reagents > Peptides and Peptidomimetics

Product Description:

Biotin-ε-aminocaproic acid N-hydroxysuccinimide ester (Biotin-X-NHS ester, CAS 72040-63-2) is a heterobifunctional crosslinking reagent. Its molecular structure consists of a biotin (vitamin H) moiety, a 6-carbon (ε-aminocaproic acid, X) spacer arm, and an amine-reactive N-hydroxysuccinimide (NHS) ester group at the terminal. This compound is primarily used as a biotinylation reagent in life science research and diagnostics. The NHS ester group reacts efficiently with primary amines (e.g., lysine residues on proteins or amine-modified nucleic acids) under mild aqueous conditions to form stable amide bonds. The extended spacer arm (X) reduces steric hindrance after conjugation, enhancing the accessibility of the biotin tag to streptavidin or avidin. This property is crucial for techniques such as ELISA, Western blotting, immunohistochemistry, affinity purification, and flow cytometry. The biotin-streptavidin interaction is one of the strongest non-covalent bonds in nature, making this reagent a fundamental tool for detecting, capturing, and isolating biomolecules. It is widely employed in the development of diagnostic assays, probe labeling, and the study of protein-protein interactions.

Physical Properties

Melting Point: 169-171 °C

Density: 1.35 g/cm3

Solubility: H2O: ≤2 mg/mL with sonication

Storage: −20°C

Analytical Data

Appearance: White solid