N-[(2S)-3-(4-hydroxyphenyl)-1-(4-nitroanilino)-1-oxopropan-2-yl]benzamide, CAS No. 6154-45-6

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6154-45-6

Catalog No. EBD133492

CAS 6154-45-6

Name N-[(2S)-3-(4-hydroxyphenyl)-1-(4-nitroanilino)-1-oxopropan-2-yl]benzamide

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Basic Information

Synonyms: N-benzoyl-L-tyrosine-P-nitroanilide Bz-Tyr-pNA Nalpha-benzoyl-N-(4-nitrophenyl)-L-tyrosinamide Nalpha-benzoyl-N-(4-nitrophenyl)tyrosinamide N-[(1S)-1-[(4-hydroxyphenyl)methyl]-2-(4-nitroanilino)-2-oxo-ethyl]benzamide N-[(2S)-3-(4-hydroxyphenyl)-1-(4-nitroanilino)-1-oxopropan-2-yl]benzamide

Molecular Formula: C22H19N3O5

Molecular Weight: 405.4

MDL Number: MFCD00038109

Categories: Medicinal Chemistry > Biochemicals and Life Science Reagents > Proteins and Enzymes Synthetic Chemistry > Organic Building Blocks > Amines

Product Description:

N-Benzoyl-L-tyrosine p-nitroanilide (Bz-Tyr-pNA, CAS 6154-45-6) is a chromogenic peptide substrate. It is a synthetic amide derivative where the N-terminal amino group of L-tyrosine is protected by a benzoyl group, and the C-terminal carboxyl group is coupled to p-nitroaniline. This compound is primarily used as a specific substrate for the detection and kinetic assay of the proteolytic enzyme α-chymotrypsin. The enzyme cleaves the amide bond between the tyrosine residue and the p-nitroaniline moiety. This hydrolysis reaction releases free p-nitroaniline, which is yellow in color and can be quantified spectrophotometrically at around 405-410 nm. This property makes it a standard tool in enzymology for studying protease activity, inhibition, and enzyme kinetics. Due to its defined structure and reactivity, it also serves as a specialized synthetic building block in peptide chemistry and biochemical research. It is a high-purity reagent intended for research use.

Physical Properties

Boiling Point: 776.8 °C at 760 mmHg

Flash Point: 423.6 °C

Density: 1.373 g/cm3

Storage: −20°C