N-tert-Butoxycarbonyl-D-threonine (Boc-D-Thr-OH) is a protected derivative of the non-essential amino acid D-threonine. It features a tert-butoxycarbonyl (Boc) group protecting the amino functionality, making it a crucial chiral building block in peptide synthesis, particularly for solid-phase peptide synthesis (SPPS) methodologies. Its primary application is in the pharmaceutical industry as a key intermediate for the synthesis of peptides and peptidomimetics. The Boc protecting group is stable under basic conditions and can be selectively removed under mild acidic conditions, allowing for sequential peptide chain elongation. The D-configuration of the threonine residue is essential for constructing peptides with specific stereochemistry, which can influence biological activity, stability against enzymatic degradation, and receptor binding affinity. This compound is a standard reagent in research laboratories focused on medicinal chemistry and biochemistry for developing novel therapeutic peptides, including antimicrobial peptides and hormone analogs. It must be stored under inert conditions to prevent deprotection or racemization.