Platyphylline is a pyrrolizidine alkaloid (PA) naturally found in plants of the *Senecio* genus, such as *Senecio platyphyllus*. It is structurally characterized by a necine base (1-methylpyrrolizidine) esterified with platynecic acid. This compound is a stereoisomer of senecionine, another common PA. Historically, platyphylline has been investigated for its pharmacological properties. It exhibits anticholinergic and spasmolytic activities, acting as a competitive antagonist at muscarinic acetylcholine receptors. These properties led to its past use in some regions as an antispasmodic agent for treating gastrointestinal and biliary tract disorders. However, like many PAs, platyphylline is hepatotoxic and potentially pneumotoxic due to metabolic activation in the liver, forming reactive pyrrolic derivatives that cause cellular damage. This significant toxicity has severely limited its modern therapeutic application. Currently, platyphylline is primarily of interest in toxicology and phytochemistry research. It serves as a reference standard for the identification and quantification of pyrrolizidine alkaloids in plant materials, herbal products, and food supplements, which is crucial for safety assessments due to the widespread contamination concerns associated with these hepatotoxic compounds.