N-(10-formyl-7,8-dihydro-pteroyl)-L-glutamic acid, commonly known as 10-formyl-7,8-dihydrofolic acid, is a derivative of folic acid (vitamin B9). It is a key intermediate in the folate metabolic pathway, specifically serving as a substrate for the enzyme 5,10-methenyltetrahydrofolate cyclohydrolase. This compound plays a crucial role in one-carbon metabolism, which is essential for the biosynthesis of purines, thymidylate, and certain amino acids. Its primary application is in biochemical and pharmaceutical research as a reference standard and a critical intermediate in studying folate metabolism and antifolate drug mechanisms. It is particularly relevant in cancer research, as antifolate drugs (like methotrexate) target this pathway to inhibit rapidly dividing cells. The compound is also used in enzymatic assays and as a starting material for synthesizing other folate analogs and coenzymes. As a sodium salt, it offers improved solubility in aqueous solutions for experimental use. Handling requires standard laboratory precautions for biochemical reagents.