(L)-(1S,2S)-N-(benzyloxycarbonyl)-O-tert-butylthreonine dicyclohexylamine salt is a protected derivative of the non-proteinogenic amino acid L-allo-threonine. Its structure features a benzyloxycarbonyl (Cbz) group protecting the amine functionality and a tert-butyl group protecting the side-chain hydroxyl group, with the molecule existing as a dicyclohexylamine (DCHA) salt to enhance stability and handling properties. The compound possesses two chiral centers in the (1S,2S) configuration, making it a valuable chiral building block. This compound is primarily employed as a key chiral intermediate in the synthesis of complex bioactive molecules, particularly in pharmaceutical research. Its main application is in the preparation of peptide-based drugs and peptidomimetics, where the protected L-allo-threonine residue is incorporated to modulate the biological activity, stability, or pharmacokinetic properties of the target compound. The specific stereochemistry (allo-configuration) is crucial for achieving the desired interaction with biological targets. The DCHA salt form improves the compound's crystallinity and facilitates purification processes. As a protected amino acid derivative, it is a standard reagent in solid-phase peptide synthesis (SPPS) and solution-phase peptide chemistry, allowing for selective deprotection and further coupling reactions.